Our Research
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We use our own quantum dots (QDs) and other fluorophores spanning broad wavelength ranges for use in Förster resonance energy transfer (FRET)-based sensing, visual color change-based sensing, and preclinical imaging.
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Nanotoxicity and degradation considerations are forefront for considering how we could better design QDs for eventual clinical translation as optical contrast agents for human diagnostic imaging.
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We are committed to devising and sharing accessible protocols for coating and biofunctionalizing QDs to promote dissemination of the materials for applications led by a diverse set of research groups.
QD Development
Our research optimizes semiconductor quantum dots (QDs) for biomedical applications through three key approaches. Firstly, we tailor QD optical properties by customizing composition and morphology to suit biomedical applications. Secondly, we study the impact of coatings and bioconjugations on nanodevice functionality, including self-assembly of biomolecules on the QD surface, reduction of non-specific binding, and modulation of in vivo circulation half-life. Thirdly, we extensively evaluate the biodegradation and biocompatibility of nanoparticle designs, with a focus on developing inorganic nanomaterials that can safely degrade for excretion.
In Vivo imaging
We leverage the brightness and color-tunability of semiconductor quantum dots for deep tissue imaging using longer wavelengths of light, specifically in the shortwave infrared (SWIR) range. We are optimizing multiple compositions of quantum dots tailored to SWIR (1000-1600 nm) emission wavelengths to mitigate light absorption and scattering by tissue, which is commonly observed in the visible (490-650 nm) and near infrared (NIR-I; 650-950 nm) regions. Using SWIR imaging, we increase depth penetration, improve resolution, and enhance visualization of biological structures.
We harness the unique properties of quantum dots (QDs) for fluorescence biosensing, utilizing Förster resonance energy transfer (FRET) or spatial localization of fluorescence to achieve sensitive detection of analytes such as pH changes, enzyme cleavage events, and small molecules. By leveraging these techniques, we enhance the accuracy and sensitivity of biosensing applications.
